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v122159 addgene 62723 aav5 ef1a dio enphr3 0 mcherry  (Addgene inc)


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    Structured Review

    Addgene inc v122159 addgene 62723 aav5 ef1a dio enphr3 0 mcherry
    V122159 Addgene 62723 Aav5 Ef1a Dio Enphr3 0 Mcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 120 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/paav+syn/pAAV-Syn-FLEX-rc%5BChrimsonR-tdTomato%5D+(Plasmid+%2362723)/pm42822450-291-41-42
    Average 96 stars, based on 120 article reviews
    v122159 addgene 62723 aav5 ef1a dio enphr3 0 mcherry - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: A sensitive soma-localized red fluorescent calcium indicator for in vivo imaging of neuronal populations at single-cell resolution
    Article Snippet: .. To clone pAAV-Syn-NES-Red-GECI, the cDNA of NES-jRGECO1a, NES-KGECO1 or XCaMP-R was PCR amplified with BamHI/EcoRI flanking site and swapped with the CoChR-GFP gene in the pAAV-Syn–CoChR–GFP (Addgene plasmid #59,070). ..

    Article Title: A sensitive soma-localized red fluorescent calcium indicator for in vivo imaging of neuronal populations at single-cell resolution.
    Article Snippet: .. To clone pAAV-Syn-NES-Red-GECI, the cDNA of NES-jRGECO1a, NES-KGECO1 or XCaMP-R was PCR amplified with BamHI/EcoRI flanking site and swapped with the CoChR-GFP gene in the pAAV-Syn–CoChR–GFP (Addgene plasmid #59,070). ..

    Article Title: A Sensitive Soma-localized Red Fluorescent Calcium Indicator for Multi-Modality Imaging of Neuronal Populations In Vivo
    Article Snippet: .. To clone pAAV-Syn-NES-Red-GECI, the cDNA of NES-jRGECO1a, NES-KGECO1 or XCaMP-R was PCR amplified with BamHI/ EcoRI flanking site and swapped with the CoChR-GFP gene in the pAAV-Syn–CoChR–GFP (Addgene plasmid #59070). ..

    Amplification:

    Article Title: A sensitive soma-localized red fluorescent calcium indicator for in vivo imaging of neuronal populations at single-cell resolution
    Article Snippet: .. To clone pAAV-Syn-NES-Red-GECI, the cDNA of NES-jRGECO1a, NES-KGECO1 or XCaMP-R was PCR amplified with BamHI/EcoRI flanking site and swapped with the CoChR-GFP gene in the pAAV-Syn–CoChR–GFP (Addgene plasmid #59,070). ..

    Article Title: A sensitive soma-localized red fluorescent calcium indicator for in vivo imaging of neuronal populations at single-cell resolution.
    Article Snippet: .. To clone pAAV-Syn-NES-Red-GECI, the cDNA of NES-jRGECO1a, NES-KGECO1 or XCaMP-R was PCR amplified with BamHI/EcoRI flanking site and swapped with the CoChR-GFP gene in the pAAV-Syn–CoChR–GFP (Addgene plasmid #59,070). ..

    Article Title: A Sensitive Soma-localized Red Fluorescent Calcium Indicator for Multi-Modality Imaging of Neuronal Populations In Vivo
    Article Snippet: .. To clone pAAV-Syn-NES-Red-GECI, the cDNA of NES-jRGECO1a, NES-KGECO1 or XCaMP-R was PCR amplified with BamHI/ EcoRI flanking site and swapped with the CoChR-GFP gene in the pAAV-Syn–CoChR–GFP (Addgene plasmid #59070). ..

    Plasmid Preparation:

    Article Title: A sensitive soma-localized red fluorescent calcium indicator for in vivo imaging of neuronal populations at single-cell resolution
    Article Snippet: .. To clone pAAV-Syn-NES-Red-GECI, the cDNA of NES-jRGECO1a, NES-KGECO1 or XCaMP-R was PCR amplified with BamHI/EcoRI flanking site and swapped with the CoChR-GFP gene in the pAAV-Syn–CoChR–GFP (Addgene plasmid #59,070). ..

    Article Title: A sensitive soma-localized red fluorescent calcium indicator for in vivo imaging of neuronal populations at single-cell resolution.
    Article Snippet: .. To clone pAAV-Syn-NES-Red-GECI, the cDNA of NES-jRGECO1a, NES-KGECO1 or XCaMP-R was PCR amplified with BamHI/EcoRI flanking site and swapped with the CoChR-GFP gene in the pAAV-Syn–CoChR–GFP (Addgene plasmid #59,070). ..

    Article Title: A Sensitive Soma-localized Red Fluorescent Calcium Indicator for Multi-Modality Imaging of Neuronal Populations In Vivo
    Article Snippet: .. To clone pAAV-Syn-NES-Red-GECI, the cDNA of NES-jRGECO1a, NES-KGECO1 or XCaMP-R was PCR amplified with BamHI/ EcoRI flanking site and swapped with the CoChR-GFP gene in the pAAV-Syn–CoChR–GFP (Addgene plasmid #59070). ..

    other:

    Article Title: Hyperactivity of the Amygdala Mediates Depressive-Like Phenotypes and Decreased Serotonin Release
    Article Snippet: The viruses utilized are as follows: AAV9-CamKiia-hM4Di-mCherry-WPRE-PA (5.33 × 1012 vg/ml), AAV9-TPH2-Cre (5.29 × 1012 vg/ml), AAV9-mDlx-GCaMP6s-WPRE-SV40 (1.00 × 1013 vg/ml) was obtained from BrainVTA company (Wuhan, China). pAAV-hSyn-EGFP (3.30×1013 vg/ml), pENN-AAV/retro-hSyn-Cre-WPRE-hGH (1.90×1013 vg/ml), pAAV9-hSyn-DIO-mCherry (2.30×1013 vg/ml), pENN-AAV1-hSyn-Cre-WPRE-hGH (1.90×1013 vg/ml), pAAV-Syn-ChrimsonR-tdT (2.60×1013 vg/ml), pAAV-Syn-GCaMP6s-WPRE-SV40 (2.70×1013 vg/ml), pAAV-hsyn-hM4D(Gi)-mCherry (2.00×1013 vg/ml), pAAV-Syn-Flex-GCaMP6s-WPRE-SV40 (2.50×1013 vg/ml) were purchased from Addgene (MA, USA).

    Injection:

    Article Title: The role of amygdala GABA neurons in controlling stress and reproduction in female mice.
    Article Snippet: .. Separate mice cohorts received the following viral injection into the MePD: (i) a mixture of pAAV-SynFlex-rc[Chrimson-tdTomato] (2.0×1012 gc/mL, Serotype:5, #62723, Addgene, Massachusetts, USA) + pAAV-EF1a-fDIO-GCaMP6s (2.3×1012 gc/mL; Serotype:8; #105715, Addgene, Massachusetts, USA; final volume 400 nl); (ii) a mixture of pAAV-Syn-Flex-rc[ChrimsontdTomato] (2.0×1012 gc/mL, Serotype:5, #62723, Addgene, Massachusetts, USA) + pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 (4.3×1012 gc/mL; Serotype:9; # 100842, Addgene, Massachusetts, USA; final volume 400 nL); (iii) a mixture of control virus pAAV-hSyn-DIO-mCherry (2.3×1012 gc/mL, Serotype:9, #50459) + pAAV-EF1a-fDIO-GCaMP6s (2.3×1012 gc/mL, Serotype:8, #105715, final volume 400nl) . .. One week after administering the virus, a 600-μm diameter, 7.2-mm long gradient index (GRIN) ProViewTM Integrated Lens (Inscopix, PN:100004554) was implanted at the same location as the viral injection.

    Article Title: Amygdala GABA Neurons: Gatekeepers of Stress and Reproduction
    Article Snippet: .. Separate mice cohorts received the following viral injection into the MePD: (i) a mixture of pAAV-Syn-Flex-rc[Chrimson-tdTomato] (2.0×10 gc/mL, Serotype:5, #62723, Addgene, Massachusetts, USA) + pAAV-EF1a-fDIO-GCaMP6s (2.3×10 gc/mL; Serotype:8; #105715, Addgene, Massachusetts, USA; final volume 400nl); (ii) a mixture of pAAV-Syn-Flex-rc[Chrimson-tdTomato] (2.0×10 gc/mL, Serotype:5, #62723, Addgene, Massachusetts, USA) + pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 (4.3×10 gc/mL; Serotype:9; # 100842, Addgene, Massachusetts, USA; final volume 400nl); (iii) a mixture of control virus AAV-Ef1a-DIO-EYFP (250nl, 4.4 × 10 gc/mL, Serotype:9, #27056, Addgene, Massachusetts, USA) + pAAV-EF1a-fDIO-GCaMP6s (2.3×10 gc/mL; Serotype:8; #105715, Addgene, Massachusetts, USA; final volume 250nl). .. One week after administering the virus, a 600-μm diameter, 7.2-mm long gradient index (GRIN) ProViewTM Integrated Lens (Inscopix, PN:100-004554) was implanted at the same location as the viral injection.

    Control:

    Article Title: The role of amygdala GABA neurons in controlling stress and reproduction in female mice.
    Article Snippet: .. Separate mice cohorts received the following viral injection into the MePD: (i) a mixture of pAAV-SynFlex-rc[Chrimson-tdTomato] (2.0×1012 gc/mL, Serotype:5, #62723, Addgene, Massachusetts, USA) + pAAV-EF1a-fDIO-GCaMP6s (2.3×1012 gc/mL; Serotype:8; #105715, Addgene, Massachusetts, USA; final volume 400 nl); (ii) a mixture of pAAV-Syn-Flex-rc[ChrimsontdTomato] (2.0×1012 gc/mL, Serotype:5, #62723, Addgene, Massachusetts, USA) + pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 (4.3×1012 gc/mL; Serotype:9; # 100842, Addgene, Massachusetts, USA; final volume 400 nL); (iii) a mixture of control virus pAAV-hSyn-DIO-mCherry (2.3×1012 gc/mL, Serotype:9, #50459) + pAAV-EF1a-fDIO-GCaMP6s (2.3×1012 gc/mL, Serotype:8, #105715, final volume 400nl) . .. One week after administering the virus, a 600-μm diameter, 7.2-mm long gradient index (GRIN) ProViewTM Integrated Lens (Inscopix, PN:100004554) was implanted at the same location as the viral injection.

    Article Title: Amygdala GABA Neurons: Gatekeepers of Stress and Reproduction
    Article Snippet: .. Separate mice cohorts received the following viral injection into the MePD: (i) a mixture of pAAV-Syn-Flex-rc[Chrimson-tdTomato] (2.0×10 gc/mL, Serotype:5, #62723, Addgene, Massachusetts, USA) + pAAV-EF1a-fDIO-GCaMP6s (2.3×10 gc/mL; Serotype:8; #105715, Addgene, Massachusetts, USA; final volume 400nl); (ii) a mixture of pAAV-Syn-Flex-rc[Chrimson-tdTomato] (2.0×10 gc/mL, Serotype:5, #62723, Addgene, Massachusetts, USA) + pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 (4.3×10 gc/mL; Serotype:9; # 100842, Addgene, Massachusetts, USA; final volume 400nl); (iii) a mixture of control virus AAV-Ef1a-DIO-EYFP (250nl, 4.4 × 10 gc/mL, Serotype:9, #27056, Addgene, Massachusetts, USA) + pAAV-EF1a-fDIO-GCaMP6s (2.3×10 gc/mL; Serotype:8; #105715, Addgene, Massachusetts, USA; final volume 250nl). .. One week after administering the virus, a 600-μm diameter, 7.2-mm long gradient index (GRIN) ProViewTM Integrated Lens (Inscopix, PN:100-004554) was implanted at the same location as the viral injection.

    Virus:

    Article Title: The role of amygdala GABA neurons in controlling stress and reproduction in female mice.
    Article Snippet: .. Separate mice cohorts received the following viral injection into the MePD: (i) a mixture of pAAV-SynFlex-rc[Chrimson-tdTomato] (2.0×1012 gc/mL, Serotype:5, #62723, Addgene, Massachusetts, USA) + pAAV-EF1a-fDIO-GCaMP6s (2.3×1012 gc/mL; Serotype:8; #105715, Addgene, Massachusetts, USA; final volume 400 nl); (ii) a mixture of pAAV-Syn-Flex-rc[ChrimsontdTomato] (2.0×1012 gc/mL, Serotype:5, #62723, Addgene, Massachusetts, USA) + pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 (4.3×1012 gc/mL; Serotype:9; # 100842, Addgene, Massachusetts, USA; final volume 400 nL); (iii) a mixture of control virus pAAV-hSyn-DIO-mCherry (2.3×1012 gc/mL, Serotype:9, #50459) + pAAV-EF1a-fDIO-GCaMP6s (2.3×1012 gc/mL, Serotype:8, #105715, final volume 400nl) . .. One week after administering the virus, a 600-μm diameter, 7.2-mm long gradient index (GRIN) ProViewTM Integrated Lens (Inscopix, PN:100004554) was implanted at the same location as the viral injection.


    Article Title: Amygdala GABA Neurons: Gatekeepers of Stress and Reproduction
    Article Snippet: .. Separate mice cohorts received the following viral injection into the MePD: (i) a mixture of pAAV-Syn-Flex-rc[Chrimson-tdTomato] (2.0×10 gc/mL, Serotype:5, #62723, Addgene, Massachusetts, USA) + pAAV-EF1a-fDIO-GCaMP6s (2.3×10 gc/mL; Serotype:8; #105715, Addgene, Massachusetts, USA; final volume 400nl); (ii) a mixture of pAAV-Syn-Flex-rc[Chrimson-tdTomato] (2.0×10 gc/mL, Serotype:5, #62723, Addgene, Massachusetts, USA) + pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 (4.3×10 gc/mL; Serotype:9; # 100842, Addgene, Massachusetts, USA; final volume 400nl); (iii) a mixture of control virus AAV-Ef1a-DIO-EYFP (250nl, 4.4 × 10 gc/mL, Serotype:9, #27056, Addgene, Massachusetts, USA) + pAAV-EF1a-fDIO-GCaMP6s (2.3×10 gc/mL; Serotype:8; #105715, Addgene, Massachusetts, USA; final volume 250nl). .. One week after administering the virus, a 600-μm diameter, 7.2-mm long gradient index (GRIN) ProViewTM Integrated Lens (Inscopix, PN:100-004554) was implanted at the same location as the viral injection.

    Recombinant:


    Software:


    Bioprocessing:

    Article Title: Amygdala GABA Neurons: Gatekeepers of Stress and Reproduction
    Article Snippet: .. Separate mice cohorts received the following viral injection into the MePD: (i) a mixture of pAAV-Syn-Flex-rc[Chrimson-tdTomato] (2.0×10 gc/mL, Serotype:5, #62723, Addgene, Massachusetts, USA) + pAAV-EF1a-fDIO-GCaMP6s (2.3×10 gc/mL; Serotype:8; #105715, Addgene, Massachusetts, USA; final volume 400nl); (ii) a mixture of pAAV-Syn-Flex-rc[Chrimson-tdTomato] (2.0×10 gc/mL, Serotype:5, #62723, Addgene, Massachusetts, USA) + pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 (4.3×10 gc/mL; Serotype:9; # 100842, Addgene, Massachusetts, USA; final volume 400nl); (iii) a mixture of control virus AAV-Ef1a-DIO-EYFP (250nl, 4.4 × 10 gc/mL, Serotype:9, #27056, Addgene, Massachusetts, USA) + pAAV-EF1a-fDIO-GCaMP6s (2.3×10 gc/mL; Serotype:8; #105715, Addgene, Massachusetts, USA; final volume 250nl). .. One week after administering the virus, a 600-μm diameter, 7.2-mm long gradient index (GRIN) ProViewTM Integrated Lens (Inscopix, PN:100-004554) was implanted at the same location as the viral injection.



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